The dilution calculator equation The Tocris dilution calculator is based on the following equation: Concentration (start) x Volume (start) = Concentration (final) x Volume (final) This equation is commonly abbreviated as: C 1V 1 = C 2V 2 An example of a dilution calculation using the Tocris dilution calculator What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution? Using the equation C 1V 1 = C 2V 2, where C 1=10 mM, C 2=50 μM, V 2=20 ml and V 1 is the unknown: • Enter 10 into the Concentration (start) box and select the correct unit (millimolar) • Enter 50 into the Concentration (final) box and select the correct unit (micromolar) • Enter 20 into the Volume (final) box and select the correct unit (milliliter) • Press calculate • The answer of 100 microliter (0.1 ml) appears in the Volume (start) box.

IC50 assays, commonly used to evaluate drug efficacy, and assay development procedures, as well as standard-curve generation, involve the serial dilution of compounds, proteins, or detection agents. Software cetak nota bon. Only official editions of the Federal Register provide legal notice to. A parallel test should be conducted. Carbonaceous biochemical oxygen demand.