We want to find the cell density of 3 without redoing the calculations with cells, and using the previously calculated cell density. The final volume is 25mL and the initial volume is 15mL, so the dilution factor is 25/15 = 1.6667 (keep all your trailing sixes for accuracy). Serial vs parallel dilution method in bod. We can now apply it to the original cell density: 0.73 / 1.6667 = 0.44 cells/mL; and we can check it using the original method: 11 cells / 25mL = 0.44 cells/mL. • We end up with still 11 cells in the beaker but they’re suspended into 40mL of water. Let’s do the calculations forward: The cell density of 1 is 11 cells / 15mL water = 0.7333 cells/mL.

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